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Purifications


DNA oligos are available as purified oligos. Purifications can be performed either by our proprietary HPSF purification method, by HPLC or by PAGE gel purification. Please note that Modified oligos, should go for HPLC purification by default.


Available purifications:

HPSF: High Purity Salt Free
Proprietary cartridge purification based on reverse phased chromatography
Cleaned from any toxic chemicals and n-x products
Quality control of each oligo by Trityl-Monitoring, OD-Measurement


HPLC: High Performance Liquid Chromatography
High purity of > 80% due to high chromatographic separation
Available for all modified and unmodified oligonucleotides
Recommended for RNA oligonucleotides
Quality control of each oligo by Trityl-Monitoring, OD-Measurement and CGE (> 60mers)


PAGE: Purification by Gel Electrophoresis
Highest available purity for longmers: > 90% purity
Recommended for unmodified oligonucleotides > 60mers
Quality control of each oligo by Trityl-Monitoring, OD-Measurement and CGE (> 60mers)




Application HPSF(High Purity Salt Free) HPLC Page
Standard PCR check mark    
Multiplex PCR check mark check mark  
Real Time PCR   check mark  
Sequencing check mark    
Genotyping / SNP   check mark  
Cloning   check mark check mark
Mutagenesis   check mark check mark
Gene Synthesis     check mark
Antisense   check mark  
Primer Extension check mark check mark  
cDNA Library Generation check mark    
End Labelling and FISH   check mark  
Northern and Southern Blotting check mark check mark  
Gel Shift Assay   check mark check mark


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