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Yield Specifications


The tables below provide guaranteed minimum yields that depend on oligo properties (length and modifications), synthesis scale, and purification method. Oligos excluded from these tables may also be ordered, but specific yields cannot be guaranteed. Minimum yields are given in units of optical density (OD), which is proportional to oligo amount (the concentration in a volume of 1 ml). For more information, see Calculations :


HPSF

Synthesis Scale1 [µmol] 0.01 0.05 0.2 1 10
Average Yields2 [OD] 5 8 15 45 400
Minimum Yields2 [OD] 1.5 3 10 35 240
Length Restriction[Bases] 5-49 5-120 5-120 5-120 5-120

  • 1 The synthesis scale indicates the initial amout of 3'-bases
  • 2 Typical yield applies to a 20mer; Calculation: 1 OD = 5 nmol = 30ug; may vary for sequences with GC content > 70%, > 3 purine streches, or strong secondary structures

HPLC/Page

Synthesis Scale1 [µmol] 0.01 0.05 0.2 1 10
Average Yields2 [OD] 5 8 15 45 400
Minimum Yields2 [OD] 1.5 3 10 35 240
Length Restriction[Bases] 5-49 5-120 5-120 5-120 5-120

  • 1 The synthesis scale indicates the initial amout of 3'-bases
  • 2 Typical yield applies to a 20mer; Calculation: 1 OD = 5 nmol = 30ug; may vary for sequences with GC content > 70%, > 3 purine streches, or strong secondary structures
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