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Re-Sequencing of Exons or Genome Fragments


Re-Sequencing of previously sequenced selected genomic regions (such as exons) to investigate correlations among sequence deviations and phenotypes.
Eurofins Genomics offers full services in 3 distinct phases from (I) primer design, primer synthesis and establishment of PCR conditions, (II) to high-throughput PCR and double strand sequencing , and (III) providing bioinformatics analyses to determine differences among references and samples.

Specifications

Phase I: Establishing PCR Strategies

  • Primer design and synthesis for all exons regions
  • Establishment of PCR amplification conditions for at least two DNA samples
  • Double strand sequencing of the PCR fragments
  • Two repetitions of PCR setup in the event of failure

Phase II: PCR and Sequencing

  • High throughput PCR of all samples
  • Purification of DNA from the PCR products
  • High throughput sequencing of PCR DNA
  • Single repetition in the event of sequencing failure

Phase III: Bioinformatic Analysis

  • Double stranded data provide strongest support for highest quality results
  • Identification of all differences among references and samples
  • Detailed identification of homozygous and/or heterozygous substitutions
  • Detection of insertions/deletions
  • Mutation report and comparison of all samples provided

Your Advantages

  • Reliable analysis of genetic variations
  • High quality results and concise analysis format
  • Fast turnaround times
  • Consultancy and project support according to your experimental needs
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Eurofins Genomics. All rights reserved.
Eurofins Genomics India Pvt Ltd | #540/1, Doddanakundi Industrial Area 2, Hoodi, Whitefield| Bangalore 560048,INDIA
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