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DNA Sequencing Sample Submission Guidelines


For rapid processing of your sequencing samples with the best results, please follow these sample submission guidelines.

All Samples and Services

Sequencing templates should be provided as purified plasmid, BAC, or PCR product Plasmid prep, PCR purification, and BAC culturing services are available, if needed Dissolve templates in water only; other buffers may inhibit the sequencing reaction Samples should be shipped overnight at room temperature

Recommended Concentrations & Volumes

  • Template Concentration Min. Volumes

    • Plasmid 150–300 ng/µl 10 µl for 1st reaction + 5 μL per add’l reaction
    • PCR <300 Bp 10–20 ng/µl 10 µl for 1st reaction + 5 μL per add’l reaction

    • PCR 300–1000 Bp 20–40 ng/µl 10 µl for 1st reaction + 5 μL per add’l reaction

    • PCR >1000 Bp 30–60 ng/µl 10 µl for 1st reaction + 5 μL per add’l reaction

    • BAC (>20) kBp 500–1000 ng/μL 10 µl for 1st reaction + 5 μL per add’l reaction

    • Primer (plasmid or PCR) 2 μM (pmol/μL) 10 µl for 1st reaction + 5 μL per add’l reaction

    • Primer (BAC) 10 μM (pmol/μL) 10 µl for 1st reaction + 5 μL per add’l reaction

    Please review your DNA sequencing order summary sheet for exact recommendations based on your specific order.

  • DNA Sequencing Templates and Primers in Tubes

  • Please use 1.7 mL microfuge tubes (for template or primer)
    Clearly label each tube for easy identification

  • DNA Sequencing Templates and Primers in Plates

  • Please use 96-well, v-bottom, full-skirt PCR plates
    Seal the plate with strip caps or a cap mat to prevent cross-contamination and sample loss
    Two wells on each plate should be left empty for our internal quality controls
    There is a 48 sample minimum per plate
    For optimal results, normalize the template concentrations across the plate to match the concentration ranges listed above

  • Premixed Samples with Primers

  • Please make template and primer solutions that match the concentration ranges listed above
    Mix 8 μL of template and 4 μL of primer for a total volume of 12 μL

  • For Ready to Load Service

  • Only reactions using ABI BigDye Terminator Kit (v3.1 or v1.1) can be accepted
    The dye terminator clean-up procedure must be performed before submitting samples

  • Ship DNA sequencing template and primers to:

  • Eurofins Genomics

    183, Gayatri Tech Park
    EPIP 2nd Phase
    Whitefield
    Bangalore


  • Primer Selection

  • Eurofins Genomics offers a wide selection of free standard primers for DNA sequencing. Below, you will find a list of those most commonly used.
    A complete listing can be found on the Standard Primer List page . You may also design your own primers or have us design, synthesize, and store your primers for an additional charge.
    If you choose to design your own primers, we recommend a length of 18–22 bases, 50–60% GC content, no more than 3 consecutive repeated bases, and an annealing temperature of 50–60°C.

  • Sample Submission for Plasmid Preparation

  • Pellet cells in standard 1.5 mL tubes or 2 mL 96-well, deep-well plates Ship overnight on dry ice

  • Sample Submission for PCR Purification

  • Dispense 10 – 50 μL of PCR product in 1.7 mL tubes or 96-well, full-skirt PCR plates
    Seal with strip caps or a cap mat
    Include a gel picture, if available
    Ship overnight at room temperature

  • Ordering DNA Sequencing Services

  • To order Eurofins MWG Operon DNA sequencing services, visit our DNA Sequencing Order Entry page.
    Follow the instructions to order DNA sequencing services for plasmids, PCR products, and BACs in tubes and 96-well plates.

If you have questions about our DNA sequencing sample submission guidelines or the online order entry system,
please call us +91 80 30706688 or send an e-mail to seqindia@eurofins.com


Sincerely,

Your Eurofins Genomics Team


Phone: +91 80 30706688 / 66
Email: seqindia@eurofins.com
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