EFFECTIVE SILENCING is more readily achieved through delivery of a guide strand
with high fidelity to the siRNA and microRNA seed regions. Our extensive quality
control procedures confirm identity of each component of the siRNA duplex: the RNAi
sense strand (passenger strand) and the RNAi antisense strand (guide strand).
You shouldn’t have to choose between the best reagents for your gene silencing experiments
and the health of your lab budget. By providing the highest quality and excellent
value, siMAX siRNA is the CLEAR CHOICE for every RNAi gene silencing experiment.
siMAX siRNA Specifications
Each siMAX siRNA duplex is provided as a quality-assured siRNA duplex with a purity
level of 90% or greater. The siRNA duplex is composed of the ‘sense’ (or ‘passenger’)
strand and the ‘antisense’ (or ‘guide’) strand. After passing all quality control
tests, 20 nmoles of both the sense and antisense RNA oligos are placed into a tube,
annealed, and then shipped as dried material to your lab.
Our siRNA are delivered as a “fully annealed” duplex. Resuspension of the dried
siRNA duplex is designed to make sure that the oligos are reannealed just prior
to use. You may use our siRNA Dilution Buffer (included free with each shipment
along with resuspension instructions) or your own preferred buffer to prepare the
siRNA duplex for futher use. All siRNA are ≥90% purity to ensure the best results
in your experiments.
To resuspend the siMAX siRNA duplex
Avoid RNA degradation by using special precautions and by wearing protective lab
clothing and gloves. All solutions should be qualified as RNAase-free and reserved
only for use in RNA experiments. All RNA work spaces must be free of RNAase contamination.
- Add 1 mL of siRNA Dilution Buffer to the lyophilized siRNA
to create a 20 uM stock solution. (siRNA Dilution Buffer, pH 8.0 is a TRIS-EDTA
buffered solution for use and/or storage of siRNA.)
- Heat the tube to 90°C for 60 - 90 sec.
- Incubate at 37°C for at least one hour.
- The siRNA duplex 20µM Stock solution is now ready for use.
- Store unused 20µM Stock solution as detailed below.