RAPD primers Kit
Genomics’s RAPD 10mer Kits contain 10mer primers specifically developed for Random
Amplified Polymorphic DNA (RAPD) analysis, a technique used for DNA fingerprinting
and genetic mapping.
In the RAPD technique, a single 10mer of arbitrary sequence is used as a primer
in PCR to amplify genomic DNA where the sequence of the DNA is completely unknown.
Genomic DNA from different individuals gives different PCR products, allowing the
identification of DNA polymorphisms that can be used to identify different individuals
or as genetic markers.
The number of PCR products generated from each genomic DNA sample depends on the
primer sequence, the genomic DNA sequence, and the genome size. Assuming that the
priming sites of a 10mer RAPD primer are randomly distributed throughout a genome,
the theoretical number of PCR products is approximately 2.5 x 10-9 x G, where G
is the size of the haploid genome in base pairs. Using this calculation, a haploid
genome of 2 x 109 bp, for example, is expected to give about 5 PCR products. This
prediction is in close agreement with experimental results.
RAPD 10mers can simply be ordered via our Ecom system either as complete kits or
as single primers.
Additionally, all relevant documentation are provided online free of charge
- Oligo Synthesis Report
- Delivery Note
- Primer length: 10 bases
- Available as single primers or complete kits
- Delivery format: lyophilised
- Quality control by: