Our handy Oligo Analysis Tool calculates molecular weight (MW), extinction coefficient
(E260), pmoles/µg, pmoles/OD and µg/OD. Also displayed for your convenience are
the % GC Content, melting temp (TM), and the reverse complement of the oligo sequence
The oligo sequence is displayed in the Oligo Analysis Plot below where the full-length
of the primer is compared against itself for self-hybridization potential. To plot
your oligo against other sequences or against shorter versions of itself, please
enter a second sequence into the Plot Sequence box and click the CALCULATE button
again. Areas of hybridization are marked with an X. Areas of 4 or more contiguous
Xs are highlighted in yellow.
If the sequence is suitable for your experiment, you can use the ADD OLIGO TO CART
button to order directly from this window.
The graph below plots your sequence against itself. Complementary bases are marked,
and primer-dimer sets are marked in bold. To plot your Oligo against a second sequence,
please enter the sequence here: