Performance of primer & probes is the key to your success
Primers and probes should consistently fulfil the performance criteria of your application.
Eurofins Genomics itself is one of the biggest oligo users. We take this advantage and closely cooperate with our other genomics labs. Trust the validated quality and performance of our Optimised Application Oligos:
- qPCR probes
Fluorescent probes for real-time PCR and FRET assays
Perfect for DNA sequencing by Sanger incl. standard primers
- NGSgrade Oligos
For any kind of NGS application, validated by our NGS lab
Our Optimised Application Oligos are scientifically proven to show highly consistent results in numerous experiments.
Find out which primer is right for you!
Customer feedbacks about our Optimised Application Oligos
Share your opinion with us by using our feedback sheet.
"We used the Cloning Oligo and the HYPUR oligos to synthetically make a gene containing several point mutations (gene synthesis using degenerated primers).
After cloning into our expression vector, we sequenced 46-48 clones. In term of internal deletion (usually missing 1 bp), we see the same rate between HYPUR and the Cloning Oligo. In our hand we had around 50-52% of complete gene with those primers. In term of diversity (the primer were degenerated), we found the best diversity with the Cloning Oligos (every mutation was represented 50-70% as expected). Using the HYPUR, we have some site which have 10 mutationà almost no diversity).
Next to that Cloning/HYPUR comparison, we also did 2 extra libraries using different set of primers (both Cloning Oligos). Here the percent of gene without deletion was much higher with 78% and 61%, and the diversity was as expected.
All together, and in addition to an higher level of synthesis, I believe that your Cloning Oligos seems to be of superior quality and are most suitable for our various application."
Christophe Blanchetot, PhD, FarGEN-X BVBA, Gent, Belgium
"For our PCR with subsequent cloning of the PCR products, using commercially available cloning vectors, we ordered new Cloning Oligos with lengths of 36, 57, 63 and 67 bases. 17 out of 25 clones were picked and subsequently sequenced at Eurofins.
All 17 clones contained the correct inserts. In summary, I found that the new Cloning Oligos performed equally as well as the purified HPSF oligos I regularly order."
Dr. Anton Schmitz, LIMES-Institut, Bonn, Germany
"For evaluation of the new Cloning Oligo quality, primer pairs for the amplification of 18 target sequences from cDNA samples were designed. All pairs were obtained twice, in the new Cloning Oligo quality as well as for reference purposes in regular HPSF purified form.The 18 target sequences were successfully amplified from the templates with both primer qualities. However the 3 reactions had to be repeated for the HPSF purified oligos with a newly synthesized cDNA preparation, to obtain a product. No problems occurred with the Cloning Oligos.
All amplifications were done with Phusion™ HF Polymerase from Finnzymes. Cloning of the amplified fragments was independently done for both primer qualities fragments into two plasmid vectors using regular techniques. After plasmid preparation from obtained E. coli transformants 2 clones from each reaction were tested for correctness of their sequence.
Surprisingly all clones amplified with the Cloning Oligos contained correct inserts without mutations due to the incorporated oligonucleotides. In contrast, clones from the HPSF purified oligos revealed deletions and mutations in 11% of the sequence runs.
This surprising result underlines the capabilities of the new primers and will allow us to reduce our running sequencing cost in the future."
Christoph R., Munich, Germany
"We tested these new cloning oligos in our gene synthesis application and got very good results. For most genes tested we found a correct clone in the first round of analysis. So, as these cloning oligos are well suited for gene synthesis, they should be almost perfect in "normal" cloning experiments."
Dr. Uwe Koehler, Eurofins Medigenomix, Ebersberg, Germany