Dye - quencher combinations for Molecular Beacons
Molecular Beacons are comparable with our Dual Labeled Probes. They are also available in a multitude of fluorescent dyes and quencher options:
|5' Reporter||Abs [nm]||Em [nm]||3' Quencher|
||TAM, BHQ1, DAB, Eclip
What you can expect from our molecular beacons
- HPLC purification included
- Wobbles (non-defined ratio) possible
- TAT of 3-4 weeks
- Delivery: lyophilised or concentration adjusted in tubes
- Quality control by OD measurement and MALDI-TOF MS
|Synthesis scale1 [µmol]||0.01||0.05||0.20||1.00|
|Minimum yield [OD]
1 The synthesis scale indicates the initial amount of 3'-bases.
Principle of molecular beacons
Molecular beacons contain a fluorescent reporter and a quencher at their 5' and 3' ends, respectively.
The sequence of these probes is designed to allow the formation of a hairpin structure in which the fluorescent dye and the quencher are in close proximity.
A molecular beacon specific for the sequence of interest is used in PCR. The probe is designed to anneal between the PCR primers.
When the probe hybridises to its target sequence in the PCR annealing step, the loop opens and the fluorescent reporter and quencher are separated, resulting in a fluorescent signal upon excitation. The amount of signal is proportional to the amount of target sequence, and is measured in real time to allow quantification of the amount of target sequence.