We recommend to reduce the thiol before using the oligo in your application:

1. Add 200 µl 10mM TCEP (solve 2,9mg TCEP-HCL in 1ml 1xTE buffer, pH 8.0) to your oligo

2. Shake it for 60 min at room temperature

3. Precipitate the oligo:

• Add 150 µl 3 M Na-Acetate [24,6 g Na-Acetate + 0,21 g Mg-Acetate in 100 ml dest. H2O]
• Fill tube with ethanol p.a.
• Shake gently
• Incubate 20 min at –20°C

4. Spin for 5 min at 13000 rpm, discard supernatant

5. Dry pellet at room temperature