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Frequently Asked Questions About Our Products And Services

Do I need a special primer design?

No special primer design is needed and there is no need to re-design existing primers or change the buffer system or DNA polymerase when using TINA.

Can TINA interfere with my general primer properties?

No. TINA enhances the thermal stability of an oligonucleotide duplex while leaving the ability to discriminate matching and mismatching oligonucleotides intact.

Does TINA work with all PCR assays?

TINA modified primers are built to perform in stressed end-point and real-time PCR assays. Assays which are already running at their optimum and the primer is not the limiting factor do not require TINA. In addition to primers, various other parameters such as large amplicon sized or high nucleic acid input can also limit your overall PCR. In such cases, a general review of the PCR protocol is required to get the PCR to perform. 

How low does TINA truly decrease the optimal primer concentration?

TINA modified primers reduce the necessary primer concentration by 30-50%.
A decrease in the optimal primer concentration reduces the likelihood of primer cross-reactivity or amplification of non-target nucleotide sequences.

How high does TINA truly increase the annealing temperature?

TINA increases the optimal annealing temperature by 3 to 8 °C. An increased annealing temperature reduces the probability of a PCR primer to anneal unspecifically thus increasing the overall PCR specificity.
An increased PCR sensitivity is also achieved due to the subsequently "relaxed" stringency of the primer annealing.

Can TINA improve the assay multiplexing capacity?

TINA modified primers reduce the primer concentration and increase the optimum annealing temperature. Both effects reduce the complexity of a multiplex assay and improve assay specifications.

How should I treat TINA labelled primers?

After you receive the TINA modified primers, please dissolve them in distilled water or Tris-buffer and leave them overnight in a refrigerator to completely dissovle. Since TINA modified primers are very hydrophobic, they require more time to dissolve after lyophilisation compared to standard DNA primers. Once the TINA modified primers are completely dissolved, they can be used just like any other DNA primer.

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