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Frequently Asked Questions About Our Products And Services

How much sample material do you need for small RNA sequencing?

Please provide us with 10-20 µg of total RNA of each sample to be analysed. The absolute minimum is 5-10 µg. However, we would encourage you to submit more sample material, if possible. It allows for more intensive QC and repetition in case of a failure.

How do you prepare small insert cDNA libraries for small RNA sequencing on HiSeq 2000?

First of all, small RNAs are isolated from total RNA by acrylamide gel purification. Thereafter, 3'- and 5'-adaptors are ligated to small RNAs and finally the sequencing library is generated by reverse transcription (RT)-PCR and PCR amplification.

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