Login / Register
0 Item(s)
Go to Cart
Place your order and get your standard genes up to 1000 bp produced in only 8 business days.
Submit your sequence of interest in electronic format and we will synthesise the respective DNA fragment in the required length (0-25 kbp and more). Every synthetic gene is cloned into a standard vector by default or into a vector of choice, if requested. 100% accuracy of gene synthesis is guaranteed and verified by DNA sequencing.
Standard genes are available in any length with moderate GC content (40-65%), without extensive repeats (>20bp) or critical hairpin structures and without extensive homopolymer stretches (>20bp).
The processing of your genes
The following steps describe The processes we take to generate your genes of interest:
We recommend our expert advice if your sequence template shows a high level of difficulties such as complex genes that contain many hairpins, repeats or other critical structures.
Using our proprietary software GENEius, the supplied amino acid or DNA sequences are optimised and adapted, e.g. for codon usage to any other organism. GENEius prevents direct or inverted repeats and hairpins and facilitates the distribution of G‘s and C‘s. Even the incorporation of "good motifs" is easily achieved with the GENEius software. Try our GENEius light and discover what our optimisation software can do for you.
After submission of your desired sequence, we start the experimental design step. This is achieved by dividing the gene in overlapping oligonucleotides for gene synthesis by using GENEius.
After design, the oligonucleotide synthesis phase begins. Our oligonucleotide synthesis facility - with more than 20 years of experience - guarantees highest quality and fast turnaround times.
Our gene synthesis lab is closely linked to the oligonucleotide production. The lab is the perfect combination of robotics and human know how. Aside from automation, our project managers, ensure best practise project layouts and stay in close touch with you if any question arise.
Based on your individual needs, your gene can be subcloned into one of our standard vectors or into any other vector of choice.
The standard vector maps can be found here. We use "a" high copy cloning vector with either ampicillin or kanamycin resistance.
More details about different vector options can be found under scales & possibilities.
Quality that you can count on: our in-house sequencing facility guarantees 100% accuracy of your synthetic gene by double-strand DNA sequencing.
For plasmid DNA preparation, a non manual mini-prep is generally performed free of charge as a standard procedure.For higher yields, just order one of our plasmid preps yielding up to 10mg of your plasmid.Endotoxin-free preparation for mega and giga scales are also available.
Read more about our options for plasmid preparation in scales & possiblities.
We provide you with a quality assurance report and a delivery note after your synthetic gene has been synthesised via Ecom, our online ordering system:
The quality assurance report contains:
Strict confidentiality is ensured by every project. The high quality of our service is overseen by a professional quality management. We are certified according to DIN EN ISO 9001:2000.
Scales & possiblities
Plasmid preparation and subcloning adapted just for you.
With our gene synthesis service, we offer a wide range of plasmid preparation scales and different options for your cloning vector.
Subcloning
Choose from several options for your cloning vector:
Your gene is normally cloned in a standard vector for free. This vector contains either ampicillin or kanamycin resistance.
Plasmid Preparation
You receive a free DNA mini prep that is carried out automatically on our robots. If the amount of DNA is insufficient, you can choose one of the scales above.
If you order either the mega or giga prep's, an endotoxin-free preparation procedure is available.
Please note: Prices for subcloning only apply to subclonings via two different sticky end restriction sites in normal plasmids up to 7 kb. If, e.g. TOPO cloning, blunt end cloning, cloning into only one site, recombinant cloning, subcloning into plasmids > 7 kb or complex plasmids, etc. is needed, a separate quote might be required. In case the subcloning does not work even after one repetition, we would send the gene subcloned into a standard cloning vector.
Are you sure to cancel ordering? Please wait until wizard is closing ...